Exposure of Artemia salinato glyphosate and bioremediation by isotherapy

Artemia salina, an ecotoxicity bioindicator, isa microcrustacean belonging to the order Anostraca. Glyphosate is an herbicide widely used to control weeds. However, its intoxication can cause serious damage to human health and the balance of the environment, given its effects as an endocrine disruptor.Objective:verify the possible protection of the artemia exposed to glyphosate by the addition of its isotherapic into water, by means of the evaluation of the behavioral and morphological features of nauplii and of the physical properties of remedies andtreated water, to elucidate the involved mechanisms. Methods:Artemia salinacysts were kept in culture bottles containing artificial seawatercontaining glyphosate at LC 10 (lethal concentration 10%), to promote hatching within 48 hours. The isotherapic preparations were inserted in each bottle in a 10% of the total water volume. Part of the nauplii was distributed in transparent tubes, being 10 nauplii per tube and 6 tubes per group, for behavioral analysis, and part were collected for a detailed morphological analysis, under an optical microscope. About 80 to 270 nauplii were analyzed per group. The reserved water was divided into aliquots for physicochemical analysis, that is, evaluation of the water dipole behavior by Cartwright ́s method.Results:Gly 6cH presented selective effects on nauplii hatching (p=0.02) and on defected/healthy ratio (p=0.001), representing some protective action. This result was dependent of the salinity of water and presented correspondence with the effects on solvatochromic dyes, indicating that charges and ions can be critical factors involved in the mechanism of action. We concluded that the use of isotherapics could be a plausible tool to reduce the environmental impact of the indiscriminate use of glyphosate, since these results can be reproduced in further studies.

Environmental parameters modulate lead chloride toxicity on Artemia salina

Different environmental conditions can influence the effects of toxic agents on living beings. Recently, a series of experiments performed in Artemia salina submitted to different kinds of intoxication have shown that both, isotherapic and succussed watercan change Artemia salina ́s bio resilience at different levels. Moreover, it seems to vary according to the circalunar cycle. Objective:To verify if circalunar phases and water agitation can modify the toxicity of lead chloride on Artemia salina in vitro. Methodology:Artemia salina cysts were exposed to seawater containing 0.04% of lead chloride (equal to EC10 or 10% effective concentration, previously determined in a pilot study) in 96-well culture plates. Thirty-six experimental repetitions were performed in four series to observe the possible effects of adding stirred water, the so-called succussed water, and the moon phases. The hatched cysts were recorded after 48 hours using a digital microscope (1000x magnification) to identify the hatching percentage and the viability and mobility of the born nauplii. Results:The exposition of cysts to PbCl2 led to an increase in the hatching rate, and it was more evident during the full moon (p = 0.00014) The addition of succussed water into the seawater medium reduced this effect to the baseline levels. An increase in mobility was seen in nauplii born from exposed cysts during the full moon (p = 0.00077), but this effect was not affected by the treatment with succussed water. Discussion:Although the effects of lead chloride EC10 on the increase of nauplii hatching were expected, two environmental variables changed the sensitivity of cysts to this harmful stimulus. The circalunar cycle varied the hatching rate according to the moon phase, even in laboratory conditions, and the addition of succussed water into the medium reduced the hatching rate, even with different intensities according to the moon phase. The organization of nano and microbubbles generated after the succussion of water could be related to this protective effect and can explain, at least partially the effects of high diluted preparations on this biological context. Conclusion:Environmental factors, such as the circalunar cycle and products of water agitation, can modulate the adaptative control of hatching in Artemia salina exposed to lead chloride at EC10.

Apocrine gland adenocarcinoma in a persian cat remission with homeopathic treatment: case report

Apocrine gland carcinomas are rare malignant neoplasms that occur in cats. Available treatment is surgical, would lead to total ablation of the external acoustic meatus and usually recurrent [1,2].Methodology:In December 2020, a homeopathic consultation of the feline, male, Persian, 13 years old, a history of the disease was reported, which started in 2015, adding up to six recurrences of tumor processes in the eyelids and recent formation in the left ear canal.The result of the histopathological examination confirmed apocrine adenocarcinoma.Prior to homeopathic treatment, tumors recurred despite surgical interventions and prophylactic treatment with trichloracetic acid.However, remaining formations were noted in the eyelids and ear canal. It has a rounded blackened shape in the upper right eyelid measuring 0.4 cm and the lower 0.2 cm, round. In the left ear canal, around 1.6 cm, in addition to 4 points scattered in the ear folds. Homeopathic treatment was started for two months with Arsenicum album30 cH, twice a day; Carcinosinum 200 cH, once a day, and complex containing Avena sativa4 cH, Echinacea angustifolia4 cH, Conium maculatum6 cH, Thuja officinalis6 cH and Silicea terra6 cH, four times a day. Every two months, the clinical picturewas reassessed, potenciesand frequencies readjusted.In a few months,there was complete remission of the tumor, recovery of welfare, and improvement in mood and appetite. The free and informed consent term was signed. There was no recurrence of tumors until May2022. Conclusion:This study proved to be effective, documented with photos and exams. Approaching a rare case may provide a new therapeutic possibility. The credibility of quality homeopathic case reports has been increasing due to methodological requirements using tools developed in recent studies.

Mercury chloride toxicity attenuation of the Brine Shrimp Artemia Salinaafter treatment with Mercurius corrosivusas isotherapic

Isotherapics preparedfromtoxic substances have been described as attenuation factors for heavy metal intoxicationin aquatic animals. Herein, Artemia salinaand mercury chloride were usedas a model to identify treatment-related bioresilience. The aim was to describe the effects of Mercurius corrosivus(MC) in different potencies on Artemia salinacyst hatching and on mercury bioavailability. Artemia salinacysts were exposed to 5.0 µg/mL of mercury chloride during the hatching phase. MC6cH, 30cH, and 200cHwere prepared and poured into artificial seawater. Different controls were used (nonchallenged cysts and challenged cysts treated with water, succussed water, and Ethilicum 1cH). Four series of nine experiments were performed for4 weeks to evaluate the percentage of cyst hatchingconsidering all moon phases. Soluble total mercury (THg) levels and precipitated mercury content were also evaluated. Solvatochromic dyes were used to check for eventual physicochemical markers of MCbiological activity. Two-way analysis of variance (ANOVA) with mixed modelswas used for evaluating the effect of different treatments andthe simultaneous influence of the moon phases on the cystshatching rate, at both observation times (24 and 48 hours).When necessary, outliers were removed, using the Tukeycriterion.Thelevel of significance αwas set at 5%. Significant delay (p<0.0001) in cyst hatching was observed after treatment with MC30cH, compared with the controls. An increase inTHg concentration in seawater (p<0.0018) and of chlorine/oxygen ratio (p<0.0001) in suspended micro-aggregateswas also seen, with possiblerelation with mercury bioavailability. Specific interaction of MC30cH with the solvatochromic dye ET33 (p<0.0017) was found. The other observed potencies of Mercurius corrosivus6 and 200 cH were not significant in relation to the observed groups.The results werepostulated as being protective effects of MC30cH on Artemia salina, by improving its bioresilience.

Conditions for health and welfare? A survey on Black women in the State of Rio de Janeiro / Condições para a saúde e o bem-estar? Inquérito sobre as mulheres negras do Estado do Rio de Janeiro

The research aims were to determine the health and welfare conditions of Black women in Rio de Janeiro. A survey was conducted to identify data related to self-image, sexual health, income, etc. The study concluded that the economic status and the internalized low self steam may compromise health and must be neutralized by affirmative actions leaded by the health professionals along with the Black women and society.

A pesquisa visa dados sobre saúde e bem-estar, particularmente os relativos à emancipação das mulheres negras. Os dados foram obtidos por meio do survey desenvolvido pelo Centro de Articulação das Populações Marginalizadas (CEAP) e Núcleo de Pesquisas, Informações e Políticas Públicas da Universidade Federal Fluminense (DataUff ) sobre as relações raciais no Estado do Rio de Janeiro, entre janeiro e março de 2000. Os dados evidenciam uma precária condição de saúde e bem-estar das mulheres negras e de suas famílias. Reforçam ainda mais o pressuposto de que o racismo é uma categoria relevante para a compreensão do processo saúde-doença na população brasileira.

Acorda Raimundo: Homens discutindoviolências e masculinidade / Wake up, Raimundo: Men discussing violence and masculinity

Este é um estudo pautado nos referenciais de gênero que tem por objetivo investigar o que pensam os homenssobre a violência doméstica de gênero e quais os valores envolvidos na construção da identidademasculina na cultura contemporânea. O trabalho foi realizado por meio de grupos de discussão com trabalhadoresde uma empresa de transportes coletivos da cidade de Porto Alegre. Foram três grupos nosquais participaram voluntariamente 10 homens adultos casados. A primeira questão lançada ao grupoproblematizava o porquê da violência conjugal. Os homens discutiram animadamente, contaram episódiosde suas vidas, assumiram a agressão às mulheres, porém, culpando-as por isso, afirmando que elas começamas brigas. Assistiram ao filme “Acorda Raimundo” que estimulou uma reflexão sobre a construção da masculinidade,os papéis de gênero e a validação homossocial do ser homem. Houve relatos de violências,brigas e traições, mas também falaram de relações conjugais pautadas no respeito e na negociação de conflitos.Ao encerrar eles avaliaram positivamente a atividade e sugeriram a continuidade do grupo na empresa.

This study is based on gender references, aiming at looking into what men think about domestic violenceand what values take part in the process of building the masculine identity in contemporary culture. Thework was done through discussion groups consisting of collective transportation workers in the city of PortoAlegre. We worked with three groups, each of 10 voluntary men, all of them married. The first questionproposed to the group problematized the reasons of marital violence. Men discussed animatedly, toldepisodes of their lives, admitted to having abused women, but put the blame on them for that, insisting that itis they, the women, who pick up fights. Then they watched the film Acorda, Raimundo… acorda! [Wake up,Raimundo, Brazil, 1990], which encouraged them to reflect about the construction of masculinity, genderroles and homosocial validation of what being a man means. Some of them reported cases involving violence,fights and unfaithfulness; on the other hand, marital relations based on respect and conflict negotiation werealso mentioned. In the end, they assessed positively the activity and suggested that the group go on meetingwithin the framework of the company.